Anti-NEDD4-2 (phospho Ser448) Antibody (A88143)

Western blot analysis of extracts of various cell lines, using Anti-NEDD4-2 (phospho Ser448) Antibody (A88143) at 1:1,000 dilution. NIH 3T3 cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37°C for 1 hour. C6 cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37°C for 1 hour. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA. Detection was with a ECL Basic Kit. Exposure time: 5s.

Western blot analysis of extracts of 293T cells, using Anti-NEDD4-2 (phospho Ser448) Antibody (A88143) at 1:1,000 dilution or NEDD4L antibody (A8085). 293T cells were treated by CIP(20uL/400ul) at 37°C for 1 hour. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA. Detection was with a ECL Basic Kit. Exposure time: 5s.