Anti-Nesprin3 Antibody (A87921)

Name: Anti-Nesprin3 Antibody
Brand: Antibodies.com
SKU: A87921

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This product is discontinued

Anti-Nesprin3 Antibody (A87921) has been discontinued and is no longer available.

View all Nesprin3 Antibodies.

Specifications

Name

Anti-Nesprin3 Antibody

Description

Rabbit polyclonal antibody to Nesprin3.

Applications

WB, ICC/IF

Dilutions

WB: 1:500-1:2,000, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 740-920 of human SYNE3 (NP_689805.3).

Sequence

LRLLEESLLSLIRNWHLQRMEVDSGKKMVFTNNIPKSGFLINPMDPIPRHRRRANLLQEEEGSHEDFSQLLRNFGQWLQVENSKLVRIIAMRTSTAEDLRTRKSKLQELEARVPEGQHLFENLLRLGPARGTSDELEDLRYQWMLYKSKLKDSGHLLTQSSPGEPTGFQKTRRWRGLGSLF

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

112 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

C14orf139, C14orf49, KASH domain-containing protein 3, KASH3, LINC00341, Nesprin-3, Nuclear envelope spectrin repeat protein 3, SYNE3

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (3)

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Western Blot - Anti-Nesprin3 Antibody (A87921)

Western blot analysis of extracts of various cell lines, using Anti-Nesprin3 Antibody (A87921) at 1:3,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

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Immunofluorescence - Anti-Nesprin3 Antibody (A87921)

Immunofluorescence analysis of HeLa cells using Anti-Nesprin3 Antibody (A87921) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).

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Immunofluorescence - Anti-Nesprin3 Antibody (A87921)

Immunofluorescence analysis of NIH/3T3 cells using Anti-Nesprin3 Antibody (A87921) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).