Mouse monoclonal [NF-05] antibody to Neurofilament H.
Specificity
The antibody recognizes a nonphosphorylated epitope of neurofilament heavy protein (NF-H), a 210 kDa intracellular structural protein of Intermediate Filament Proteins family. NF-H is mainly expressed in the central and peripheral nervous system and reproductive system and is biochemically very stable.
Applications
WB, IHC-Fr, ELISA
Dilutions
ELISA: This antibody has been tested as the capture antibody, WB: 1-2 µg/ml.
Reactivity
Human, Rat, Porcine
Cross Reactivity
This antibody does not cross react with Mouse.
Immunogen
Pig brain neurofilament protein-enriched fraction after depolymerization of microtubules.
Host
Mouse
Clonality
Monoclonal
Clone ID
NF-05
Isotype
IgG1
Conjugate
Unconjugated
Purification
Purified by differential precipitation and solid-phase chromatography.
Concentration
1 mg/ml
Predicted MW
210 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Western blotting analysis of human neurofilament H protein using Anti-Neurofilament H Antibody [NF-05] (A85669) on lysates of HEK-293 cell line, human brain lysate, and MCF-7 cell line (neurofilament non-expressing cell line; negative control) under reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse anti-neurofilament H monoclonal antibody followed by IRDye800-conjugated anti-mouse secondary antibody. Neurofilament H was detected around 180 kDa.
Western blotting analysis of neurofilament heavy protein in porcine brain lysate (reducing conditions) using Anti-Neurofilament H Antibody [NF-05] (A85669).
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