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Anti-Neurogenin3/NGN-3 Antibody (A11561)

Western Blot - Anti-Neurogenin3/NGN-3 Antibody (A11561) - Antibodies.com Immunohistochemistry - Anti-Neurogenin3/NGN-3 Antibody (A11561) - Antibodies.com
$355
Product Datasheet

Rabbit polyclonal antibody to Neurogenin3/NGN-3 for WB and IHC.

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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 7-10 business days.

  • Specifications

Name
Anti-Neurogenin3/NGN-3 Antibody
Description
Rabbit polyclonal antibody to Neurogenin3/NGN-3.
Applications
WB, IHC
Dilutions
WB: 1:500-1:1,000, IHC: 1:50-1:200
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-214 of human NEUROG3 (NP_066279.2).
Sequence
MTPQPSGAPTVQVTRETERSFPRASEDEVTCPTSAPPSPTRTRGNCAEAEEGGCRGAPRKLRARRGGRSRPKSELALSKQRRSRRKKANDRERNRMHNLNSALDALRGVLPTFPDDAKLTKIETLRFAHNYIWALTQTLRIADHSLYALEPPAPHCGELGSPGGSPGDWGSLYSPVSQAGSLSPAASLEERPGLLGATFSACLSPGSLAFSDFL
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Concentration
Lot Specific
Molecular Weight
25 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (2)

Western Blot - Anti-Neurogenin3/NGN-3 Antibody (A11561) - Antibodies.comEnlarge Image

Western Blot - Anti-Neurogenin3/NGN-3 Antibody (A11561)

Western blot analysis of various lysates, using Anti-Neurogenin3/NGN-3 Antibody (A11561) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 60s.
Immunohistochemistry - Anti-Neurogenin3/NGN-3 Antibody (A11561) - Antibodies.comEnlarge Image

Immunohistochemistry - Anti-Neurogenin3/NGN-3 Antibody (A11561)

Immunohistochemistry analysis of paraffin-embedded human colon tissue using Anti-Neurogenin3/NGN-3 Antibody (A11561) at a dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
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