Western Blot - Anti-Neuroligin 4 Antibody [S98-7] (A304804)
Western blot analysis of mouse brain membrane showing detection of ~125-130 kDa Neuroligin 4 protein using Anti-Neuroligin 4 Antibody [S98-7] (A304804) at 1:1,000 for 16 hours at 4°C. Lane 1: Molecular Weight Ladder (MW). Lane 2: mouse brain Membrane. Load: 15 µg. Block: 2% BSA and 2% Skim Milk in 1X TBST. The secondary antibody used was Goat Anti-Mouse IgG: HRP at 1:2000 for 60 minutes at room temperature. Color Development: ECL solution for 6 min at room temperature. Predicted/Observed Size: ~125-130 kDa.
Immunohistochemistry analysis of mouse dentate gyrus, fixed in formaldehyde and paraffin embedded. The Primary Antibody used was Anti-Neuroligin 4 Antibody [S98-7] (A304804) at 1:100. The secondary antibody used was FITC Goat Anti-Mouse (green). Counterstain: DAPI (blue) nuclear stain at 1:1,000.
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