Rabbit polyclonal antibody to Neurotensin Receptor 2.
Specificity
This antibody binds specifically to the C-terminal domain of human Neurotensin Receptor 2, detecting the non-phosphorylated isoform. It is validated for Western blot analysis, facilitates immunoprecipitation from cell and tissue lysates, and supports immunohistochemistry in cultured cells and tissue sections.
Applications
WB, ICC, IHC
Dilutions
WB: 1,000, IHC: 1:100, ICC: 1:200
Reactivity
Human
Immunogen
Synthetic peptide corresponding to the C-terminal of human Neurotensin Receptor 2 (amino acids. 390-410). Immunogen range is 22-22 amino acids.
Sequence
PQSPTLMDTASGFGDPPETRT
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Purification
Antigen affinity purification.
Concentration
Lot Specific
Product Form
Liquid
Formulation
Supplied in Dulbecco's PBS, pH 7.4, with 150 mM NaCl and 0.005% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
Levocabastine-sensitive neurotensin receptor, Neurotensin receptor type 2, NT-R-2, NTR2, NTSR2
Detection of Neurotensin Receptor 2 (Neurotensin Receptor 2) in gastric mucosa by immunohistochemistry. Gastric mucosa sections were dewaxed, microwaved in citric acid, and incubated with anti-Neurotensin Receptor 2 antibody (A334480) at 1:100, followed by biotinylated anti-rabbit IgG and avidin-biotin solution. Sections were developed with 3,3-diaminobenzidine (DAB)-glucose oxidase and lightly counterstained with hematoxylin. Neurotensin Receptor 2 receptors were uniformly observed at the plasma membrane of parietal cells.
Localization of Neurotensin Receptor 2 (Neurotensin Receptor 2) in exocrine pancreas by immunohistochemistry. Pancreatic sections were dewaxed, microwaved in citric acid, and stained with anti-Neurotensin Receptor 2 antibody (A334480) at 1:100, followed by biotinylated anti-rabbit IgG and avidin-biotin solution. Sections were developed with 3,3-diaminobenzidine (DAB)-glucose oxidase and lightly counterstained with hematoxylin. Neurotensin Receptor 2 receptors were predominantly detected at the plasma membrane of exocrine pancreatic cells.
Immunocytochemical analysis of Neurotensin Receptor 2 (Neurotensin Receptor 2) in HEK293 cells. HEK293 cells stably expressing Neurotensin Receptor 2 were untreated or treated with 1 µM Neurotensin for 30 min and stained with anti-Neurotensin Receptor 2 antibody (A334480) at 1:200. Neurotensin Receptor 2 receptors were localized to the plasma membrane in untreated cells (0 min) and translocated to the cytosol after 30 min of Neurotensin exposure.
Western blot confirmation of Neurotensin Receptor 2 (Neurotensin Receptor 2) in transfected HEK293 cells. Lysates from native HEK293 cells (MOCK) or cells stably expressing Neurotensin Receptor 2 were probed with anti-Neurotensin Receptor 2 antibody (A334480) at 1:1000.
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