Anti-Neutrophil Elastase Antibody [ARC1364] (A309860)

Name: Anti-Neutrophil Elastase Antibody [ARC1364]
Brand: Antibodies.com
SKU: A309860
Price: 325.0 GBP
Availability: InStock

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$485

Rabbit monoclonal [ARC1364] antibody to Neutrophil Elastase for WB, IHC, ICC/IF and IP.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-Neutrophil Elastase Antibody [ARC1364]

Description

Rabbit monoclonal [ARC1364] antibody to Neutrophil Elastase.

Applications

WB, IHC, ICC/IF, IP

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200, IP: 1:50-1:200

Reactivity

Human, Mouse

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 167-267 of human Neutrophil Elastase (ELANE) (P08246).

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC1364

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

30 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

Bone marrow serine protease, ELA2, ELANE, Elastase-2, HLE, Human leukocyte elastase, Medullasin, PMN elastase

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (3)

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Western Blot - Anti-Neutrophil Elastase Antibody [ARC1364] (A309860)

Western blot analysis of extracts of various cell lines, using Anti-Neutrophil Elastase Antibody [ARC1364] (A309860) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3s.

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Immunohistochemistry - Anti-Neutrophil Elastase Antibody [ARC1364] (A309860)

Immunohistochemistry analysis of paraffin-embedded mouse bone marrow using Anti-Neutrophil Elastase Antibody [ARC1364] (A309860) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Immunofluorescence - Anti-Neutrophil Elastase Antibody [ARC1364] (A309860)

Immunofluorescence analysis of human spleen using Anti-Neutrophil Elastase Antibody [ARC1364] (A309860) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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