Rabbit polyclonal antibody to NFAT5 / TonEBP (Phospho-Ser155)
Specificity
The antibody detects endogenous levels of NFAT5 / TonEBP only when phosphorylated at serine 155.
Applications
WB
Reactivity
Human, Mouse
Immunogen
Peptide sequence around phosphorylation site of serine 155 (D-N-S(p)-R-M) derived from Human NFAT5 / TonEBP.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1 mg/ml
Formulation
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage
Store at -20˚C
Synonyms
KIAA0827, NF-AT5, Nuclear factor of activated T-cells 5, T-cell transcription factor NFAT5, TonE-binding protein, TONEBP, Tonicity-responsive enhancer-binding protein
Western blot analysis of extracts from HeLa cells, treated with forskolin (40nM, 30mins), using NFAT5/TonEBP (Phospho-Ser155) antibody #12146. The lane on the right is treated with the synthesized peptide.
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