Rabbit polyclonal antibody to NF?B-p105 / p50 (Phospho-Ser337)
Specificity
The antibody detects endogenous level of NFκB-p105 / p50 only when phosphorylated at serine 337.
Applications
WB, IHC, ICC/IF
Reactivity
Human, Mouse, Rat
Immunogen
Peptide sequence around phosphorylation site of serine 337(R-K-S(p)-D-L) derived from Human NF?B-p105 / p50.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1.0 mg/mL
Predicted MW
50,120kd
Formulation
Supplied in phosphate-buffered saline (without Mg²? and Ca²?), pH 7.4, containing 150 mM NaCl, 0.02% sodium azide, and 50% glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Western blot analysis of extracts from Hela cells, treated with Noc or calf intestinal phosphatase (CIP), using NFκB-p105/p50 (Phospho-Ser337) Antibody #11017.
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