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Anti-NFkB p100/NFKB2 (phospho Ser866) Antibody (A16640)

Western Blot - Anti-NFkB p100/NFKB2 (phospho Ser866) Antibody (A16640) - Antibodies.com Immunohistochemistry - Anti-NFkB p100/NFKB2 (phospho Ser866) Antibody (A16640) - Antibodies.com Immunofluorescence - Anti-NFkB p100/NFKB2 (phospho Ser866) Antibody (A16640) - Antibodies.com
$485
Product Datasheet

Rabbit polyclonal antibody to NFkB p100/NFKB2 (phospho Ser866) for WB, IHC and ICC/IF.

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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 7-10 business days.

  • Specifications

Name
Anti-NFkB p100/NFKB2 (phospho Ser866) Antibody
Description
Rabbit polyclonal antibody to NFkB p100/NFKB2 (phospho Ser866).
Applications
WB, IHC, ICC/IF
Dilutions
WB: 1:500-1:2,000, IHC: 1:50-1:100, ICC/IF: 1:50-1:100
Reactivity
Human, Mouse
Immunogen
A synthetic phosphorylated peptide around S866 of human NF-?B2 (NP_001070962.1).
Sequence
EDSAY
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Concentration
Lot Specific
Molecular Weight
120 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (3)

Western Blot - Anti-NFkB p100/NFKB2 (phospho Ser866) Antibody (A16640) - Antibodies.comEnlarge Image

Western Blot - Anti-NFkB p100/NFKB2 (phospho Ser866) Antibody (A16640)

Western blot analysis of extracts of MDA-MB435 cells, using Anti-NFkB p100/NFKB2 (phospho Ser866) Antibody (A16640) at dilution. MDA-MB435 cells were treated by TNF-alpha. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA.
Immunohistochemistry - Anti-NFkB p100/NFKB2 (phospho Ser866) Antibody (A16640) - Antibodies.comEnlarge Image

Immunohistochemistry - Anti-NFkB p100/NFKB2 (phospho Ser866) Antibody (A16640)

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using Anti-NFkB p100/NFKB2 (phospho Ser866) Antibody (A16640). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunofluorescence - Anti-NFkB p100/NFKB2 (phospho Ser866) Antibody (A16640) - Antibodies.comEnlarge Image

Immunofluorescence - Anti-NFkB p100/NFKB2 (phospho Ser866) Antibody (A16640)

Immunofluorescence analysis of methanol-fixed HeLa cells using Anti-NFkB p100/NFKB2 (phospho Ser866) Antibody (A16640).
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