Immunohistochemistry analysis of rat colon, fixed in formalin and paraffin-embedded. The Primary Antibody used was Anti-NHE3 Antibody (A305212) at 1:25 for 1 hour at room temperature. The secondary antibody used was Goat Anti-Rabbit IgG:Alexa Fluor 488. Counterstain: Actin-binding Phalloidin-Alexa Fluor 633; DAPI (blue) nuclear stain. Magnification: 63X.(A) DAPI (blue) nuclear stain. (B) Phalloidin Alex Fluor 633 F-Actin stain. (C) NHE3 Antibody (D) Composite
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