Anti-NHEJ1 Antibody (A11799)

This product is discontinued

Anti-NHEJ1 Antibody (A11799) has been discontinued and is no longer available.

View all NHEJ1 Antibodies.

Specifications

Name

Anti-NHEJ1 Antibody

Description

Rabbit polyclonal antibody to NHEJ1.

Applications

WB, IHC, IF

Dilutions

WB: 1:200-1:2000, IHC: 1:50-1:200, IF:1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-299 of human NHEJ1 (NP_079058.1).

Sequence

MEELEQGLLMQPWAWLQLAENSLLAKVFITKQGYALLVSDLQQVWHEQVDTSVVSQRAKELNKRLTAPPAAFLCHLDNLLRPLLKDAAHPSEATFSCDCVADALILRVRSELSGLPFYWNFHCMLASPSLVSQHLIRPLMGMSLALQCQVRELATLLHMKDLEIQDYQESGATLIRDRLKTEPFEENSFLEQFMIEKLPEACSIGDGKPFVMNLQDLYMAVTTQEVQVGQKHQGAGDPHTSNSASLQGIDSQCVNQPEQLVSSAPTLSAPEKESTGTSGPLQRPQLSKVKRKKPRGLFS

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

38kDa

Formulation

Supplied in Phosphate Buffered Saline, pH 7.30, with 0.02% Sodium Azide and 50% Glycerol.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Non-homologous end-joining factor 1, Protein cernunnos, XRCC4-like factor

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (5)

Western Blot - Anti-NHEJ1 Antibody (A11799)

Western blot analysis of extracts of various cell lines, using Anti-NHEJ1 Antibody (A4985) at 1:1,000 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L) (HRP) (AS014) at 1:10,000 dilution. Lysates / proteins: 25µg per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.

Immunohistochemistry - Anti-NHEJ1 Antibody (A11799)

Immunohistochemistry of paraffin-embedded human breast cancer using Anti-NHEJ1 Antibody (A4985) at dilution of 1:100 (40x lens).

Immunohistochemistry - Anti-NHEJ1 Antibody (A11799)

Immunohistochemistry of paraffin-embedded human uterine cancer using Anti-NHEJ1 Antibody (A4985) at dilution of 1:100 (40x lens).

Immunofluorescence - Anti-NHEJ1 Antibody (A11799)

Immunofluorescence analysis of C6 cells using Anti-NHEJ1 Antibody (A4985) at dilution of 1:100. Blue: DAPI for nuclear staining.

Show more images

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