Rabbit polyclonal antibody to Niban (Phospho-Ser602)
Specificity
The antibody detects endogenous level of Niban only when phosphorylated at serine 602.
Applications
WB
Dilutions
Western blotting: 1:500~1:1000
Reactivity
Human
Immunogen
Peptide sequence around phosphorylation site of serine 602 (R-A-S(p)-A-I) derived from Human Niban.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1 mg/ml
Predicted MW
150kd
Formulation
Supplied at 1.0mg / mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage
Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Synonyms
C1orf24, Cell growth-inhibiting gene 39 protein, NIBAN1, Protein FAM129A, Protein Niban 1
Western blot analysis in vitro kinase assays were performed by mixing purified bacterially expressed WT His–Niban or the His–Niban S602A with or without purified active AKT1 using Niban (Phospho-Ser602) Antibody #11578.
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