Recombinant human monoclonal antibody to Cannabinoid Receptor I.
Applications
ELISA, FACS, Functional Assay, In Vivo
Reactivity
Human
Host
Human
Clonality
Monoclonal
Isotype
IgG4
Light Chains
kappa
Conjugate
Unconjugated
Purification
Protein A affinity chromatography.
Concentration
Reconstitution dependent.
Molecular Weight
This antibody has a predicted MW of 145 kDa.
Purity
> 95% (by SDS-PAGE and SEC-HPLC).
Product Form
Lyophilized
Reconstitution
Reconstitute with 100µl of sterile double-distilled water to bring antibody to 1mg/ml concentration. Gently shake to solubilize completely. Do not vortex!
Formulation
Lyophilized from 25mM Histadine, pH 6.2, with 8% Sucrose and 0.01% Tween80.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
SDS-PAGE analysis of Nimacimab Biosimilar - Anti-Cannabinoid Receptor I Antibody - Low endotoxin, Azide free (A323607) under reducing (R) conditions confirms that the purity of this antibody is greater than 95%.
SEC-HPLC analysis of Nimacimab Biosimilar - Anti-Cannabinoid Receptor I Antibody - Low endotoxin, Azide free (A323607) confirms that the purity of this antibody is 99.42%.
Immobilized recombinant human CB1 VLP protein at 5 µg/ml (30 uL/well) is bound by Nimacimab Biosimilar - Anti-Cannabinoid Receptor I Antibody - Low endotoxin, Azide free (A323607). EC50 was approximately 0.04092 µg/ml.
Human CB1 / CNR1 EGFP HEK293 Cell Line were stained with Nimacimab Biosimilar - Anti-Cannabinoid Receptor I Antibody - Low endotoxin, Azide free (A323607) and negative control protein respectively, washed and then followed by APC and analyzed with FACS, EC50 = 0.028 µg/ml.
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