Anti-NKCC1 Antibody (A80631)

Name: Anti-NKCC1 Antibody
Brand: Antibodies.com
SKU: A80631
Price: 270.0 GBP
Availability: InStock

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$355

Rabbit polyclonal antibody to NKCC1 for WB and IHC.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-NKCC1 Antibody

Description

Rabbit polyclonal antibody to NKCC1.

Applications

WB, IHC

Dilutions

WB: 1:100-1:500, IHC: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 201-280 of human SLC12A2 (NP_001037.1).

Sequence

YDTHTNTYYLRTFGHNTMDAVPRIDHYRHTAAQLGEKLLRPSLAELHDELEKEPFEDGFANGEESTPTRDAVVTYTAESK

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

160 - 200 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

Basolateral Na-K-Cl symporter, Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 2, SLC12A2, Solute carrier family 12 member 2

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (4)

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Western Blot - Anti-NKCC1 Antibody (A80631)

Western blot analysis of extracts of various cell lines, using Anti-NKCC1 Antibody (A80631) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Immunohistochemistry - Anti-NKCC1 Antibody (A80631)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-NKCC1 Antibody (A80631) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Immunohistochemistry - Anti-NKCC1 Antibody (A80631)

Immunohistochemistry analysis of paraffin-embedded mouse kidney using Anti-NKCC1 Antibody (A80631) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Immunohistochemistry - Anti-NKCC1 Antibody (A80631)

Immunohistochemistry analysis of paraffin-embedded rat kidney using Anti-NKCC1 Antibody (A80631) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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