Western blot analysis of extracts of LNCaP cells, using Anti-Nkx3.1 Antibody (A307522) at 1:400 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.
Western blot analysis of extracts of Mouse kidney, using Anti-Nkx3.1 Antibody (A307522) at 1:400 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.
Immunofluorescence analysis of LNCaP cells using Anti-Nkx3.1 Antibody (A307522) at a dilution of 1:20 (40x lens). DAPI was used to stain the cell nuclei (blue).
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