Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
Caterpiller protein 11.3, CLR11.3, NLR family member X1, NOD5, NOD9, Nucleotide-binding oligomerization domain protein 5, Nucleotide-binding oligomerization domain protein 9
Western blot analysis of extracts of various cell lines, using Anti-NLRX1 Antibody (A87814) at 1:3,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.
Immunoprecipitation analysis of 600µg extracts of mouse kidney using 3µg of Anti-NLRX1 Antibody (A87814). This Western blot was performed on the immunoprecipitate using Anti-NLRX1 Antibody (A87814) at a dilution of 1:1000.
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