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Anti-NOS1 Antibody [J23] (A304971)

Western Blot - Anti-NOS1 Antibody [J23] (A304971) - Antibodies.com Immunocytochemistry/Immunofluorescence - Anti-NOS1 Antibody [J23] (A304971) - Antibodies.com
$520
Product Datasheet

Mouse monoclonal [J23] antibody to NOS1 for WB, ICC/IF, ELISA and IHC.

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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 5-8 business days.

  • Specifications

Name
Anti-NOS1 Antibody [J23]
Description
Mouse monoclonal [J23] antibody to NOS1.
Applications
WB, ICC/IF, ELISA, IHC
Dilutions
WB: 1:1,000
Reactivity
Human, Mouse, Rat
Immunogen
Partial Human Recombinant NOS1 Protein.
Host
Mouse
Clonality
Monoclonal
Clone ID
J23
Isotype
IgG2a
Conjugate

Unconjugated

Purification
Protein A purification.
Concentration
1 mg/ml
Molecular Weight
~160.97 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 50% Glycerol and 0.09% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
2310005C01Rik, BNOS, Constitutive NOS, EC 1.14.13.39, IHPS 1, IHPS1, N-NOS, NC-NOS, neuronal Nitric Oxide Synthase, Neuronal NOS, Nitric oxide synthase , neuronal, included, Nitric oxide synthase 1, Nitric oxide synthase 1 (neuronal), Nitric oxide synthase, brain, Nitric oxide synthase, penile neuronal, included, NNOS, nNOS (neuronal), NO, NOS, NOS 1, NOS type I, NOS-I, NOS1_HUMAN, Peptidyl-cysteine S-nitrosylase NOS1
Isotype Controls
Suitable Secondaries
See all Anti-Mouse IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (2)

Western Blot - Anti-NOS1 Antibody [J23] (A304971) - Antibodies.comEnlarge Image

Western Blot - Anti-NOS1 Antibody [J23] (A304971)

Western blot analysis of mouse Cerebellum showing detection of NOS1/ nNos protein using Anti-NOS1 Antibody [J23] (A304971) at 1:1,000 for 2 hours at room temperature with shaking. Lane 1: MW ladder. Lane 2: mouse brain Cerebellum (7.5 ug). Load: 7.5 ug. Block: 5% Skim Milk powder in TBST. The secondary antibody used was Goat anti-mouse IgG:HRP at 1:4,000 for 1 hour at room temperature with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 minutes in room temperature.
Immunocytochemistry/Immunofluorescence - Anti-NOS1 Antibody [J23] (A304971) - Antibodies.comEnlarge Image

Immunofluorescence - Anti-NOS1 Antibody [J23] (A304971)

Immunocytochemistry/Immunofluorescence analysis of human differentiated SH-SY5Y, using Anti-NOS1 Antibody [J23] (A304971), at 1:250. The secondary antibody used was AlexaFluor 488. Counterstain: phalloidin (Alexa 647, red), beta tubulin (Anti-beta III Tubulin Ab, Alexa 555, magenta) Hoechst (blue).(A) Phalloidin (B) Anti-beta III Tubulin Ab. (C) NOS1/nNOS Antibody. (D) Hoechst (E) Composite.
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