This antibody recognizes intracellular domain of Notch 1 protein, mainly its activated form. The unprocessed Notch 1 protein is recognized with lower affinity.
GST fusion protein containing cdc10-NCR region of mouse Notch1.
Host
Mouse
Clonality
Monoclonal
Clone ID
mN1A
Isotype
IgG1
Light Chains
kappa
Conjugate
PE
Excitation: 565nm, Emission: 578nm
Purification
This antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography; removing unconjugated antibody and free fluorochrome.
Concentration
100 µg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Store in the dark at 2-8°C. Do not freeze!
Synonyms
hN1, Neurogenic locus notch homolog protein 1, Notch 1, TAN1, Translocation-associated notch protein TAN-1
Flow cytometry multicolor surface staining of human PHA stimulated CD3 positive lymphocytes using Anti-IL-2 Receptor alpha Antibody [MEM-181] (APC) (A85771), (10 µl reagent / 100 µl of sample) and intracellular staining of human lymphocytes using Anti-Notch1 Antibody [mN1A] (PE) (A86360), (concentration in sample 3 µg/ml).
Separation of CD3 positive CD25 positive cells stained with Anti-Notch1 Antibody [mN1A] (PE) (A86360), (concentration in sample 3 µg/ml, red-filled) from CD3 positive CD25 positive cells stained using Mouse IgG1 kappa Isotype Control [MOPC-21] (PE) (A86199), (concentration in sample 3 µg/ml, same as Notch1 PE concentration, black-dashed) in flow cytometry analysis (intracellular staining) of PHA stimulated human peripheral whole blood .
Flow cytometry intracellular staining of PHA stimulated human peripheral whole blood staining Notch1 with Anti-Notch1 Antibody [mN1A] (PE) (A86360), (concentration in sample 3 µg/ml, left) or Mouse IgG1 kappa Isotype Control [MOPC-21] (PE) (A86199), (concentration in sample 3 µg/ml, same as Notch1 PE concentration, right).
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