Anti-NQO1 Antibody [ARC56753] (A307961)

Western blot analysis of extracts of various lysates, using Anti-NQO1 Antibody (A307961) at 1:20,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

Western blot analysis of extracts from wild type(WT) and NQO1 knockdown (KD) HeLa cells, using Anti-NQO1 Antibody (A307961) at 1:20,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

Immunohistochemistry analysis of paraffin-embedded mouse kidney using Anti-NQO1 Antibody (A307961) at dilution of 100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.0 before commencing with IHC staining protocol.

Immunofluorescence analysis of rat stomach using Anti-NQO1 Antibody (A307961) at a dilution of 1:300 (40x lens). DAPI was used to stain the cell nuclei (blue).