Western blot analysis of extracts of HepG2 cells, using Anti-NRBF2 Antibody (A9878) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3min.
Western blot analysis of extracts from normal (control) and NRBF2 knockout (KO) 293T cells, using Anti-NRBF2 Antibody (A9878) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.
Immunohistochemistry analysis of paraffin-embedded human esophageal using Anti-NRBF2 Antibody (A9878) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunofluorescence analysis of C6 cells using Anti-NRBF2 Antibody (A9878) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunofluorescence analysis of HeLa cells using Anti-NRBF2 Antibody (A9878) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunofluorescence analysis of NIH-3T3 cells using Anti-NRBF2 Antibody (A9878) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
