Anti-NSUN6 Antibody (A12091)

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This product is discontinued

Anti-NSUN6 Antibody (A12091) has been discontinued and is no longer available.

View all NSUN6 Antibodies.

Specifications

Name

Anti-NSUN6 Antibody

Description

Rabbit polyclonal antibody to NSUN6.

Applications

WB, IP

Dilutions

WB: 1:500-1:2000, IP: 1:50-1:200

Reactivity

Human, Mouse

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 220-469 of human NSUN6 (NP_872349.1).

Sequence

NLPSALVSHVLNPQPGEKILDLCAAPGGKTTHIAALMHDQGEVIALDKIFNKVEKIKQNALLLGLNSIRAFCFDGTKAVKLDMVEDTEGEPPFLPESFDRILLDAPCSGMGQRPNMACTWSVKEVASYQPLQRKLFTAAVQLLKPEGVLVYSTCTITLAENEEQVAWALTKFPCLQLQPQEPQIGGEGMRGAGLSCEQLKQLQRFDPSAVPLPDTDMDSLREARREDMLRLANKDSIGFFIAKFVKCKST

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

52kDa

Formulation

Supplied in Phosphate Buffered Saline, pH 7.30, with 0.02% Sodium Azide and 50% Glycerol.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

NOL1/NOP2/Sun and PUA domain-containing protein 1, NOL1/NOP2/Sun domain family member 6, NOPD1, tRNA (cytosine(72)-C(5))-methyltransferase NSUN6

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (2)

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Western Blot - Anti-NSUN6 Antibody (A12091)

Western blot analysis of extracts of various cell lines, using Anti-NSUN6 Antibody (A7205) at 1:1,000 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L) (HRP) (AS014) at 1:10,000 dilution. Lysates / proteins: 25µg per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 40s.

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Immunoprecipitation - Anti-NSUN6 Antibody (A12091)

Immunoprecipitation analysis of 200µg extracts of HeLa cells using 1µg Anti-NSUN6 Antibody (A7205). Western blot was performed from the immunoprecipitate using Anti-NSUN6 Antibody (A7205) at a dilition of 1:1000.