At-T20 cell lysate detected with Anti-Nucleolin Antibody at a 1:2,500 dilution. Lysate at 50µg and rabbit anti-goat IgG antibody (HRP) at a 1:10,000 dilution.
Quantitative immunofluorescence analyses for the presence SCNG+ RGCs and CRX+ photoreceptor precursors. Bottom panel inset panels show individual antibody and nuclear staining. White arrowhead show the presence of rosettes comprised of Ki67+ proliferating cells. Scale bars 50?µM.
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