Anti-Nucleoside-diphosphate kinase Antibody (A15278)

Name: Anti-Nucleoside-diphosphate kinase Antibody
Brand: Antibodies.com
SKU: A15278
Price: 270.0 GBP
Availability: InStock

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$355

Rabbit polyclonal antibody to Nucleoside-diphosphate kinase for WB, ICC/IF and IP.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-Nucleoside-diphosphate kinase Antibody

Description

Rabbit polyclonal antibody to Nucleoside-diphosphate kinase.

Applications

WB, ICC/IF, IP

Dilutions

WB: 1:500-1:2,000, ICC/IF: 1:50-1:200, IP: 1:500-1:1,000

Reactivity

Human, Mouse

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 69-228 of human CMPK1 (NP_057392.1).

Sequence

LLRDERKNPDSQYGELIEKYIKEGKIVPVEITISLLKREMDQTMAANAQKNKFLIDGFPRNQDNLQGWNKTMDGKADVSFVLFFDCNNEICIERCLERGKSSGRSDDNRESLEKRIQTYLQSTKPIIDLYEEMGKVKKIDASKSVDEVFDEVVQIFDKEG

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

22 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

CK, CMK, CMPK, dCMP kinase, Deoxycytidylate kinase, UCK, UMK, UMP-CMP kinase, UMP/CMP kinase, UMP/CMPK, UMPK, Uridine monophosphate/cytidine monophosphate kinase

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (3)

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Western Blot - Anti-Nucleoside-diphosphate kinase Antibody (A15278)

Western blot analysis of extracts of various cell lines, using Anti-Nucleoside-diphosphate kinase Antibody (A15278) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

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Immunofluorescence - Anti-Nucleoside-diphosphate kinase Antibody (A15278)

Immunofluorescence analysis of MCF7 cells using Anti-Nucleoside-diphosphate kinase Antibody (A15278). DAPI was used to stain the cell nuclei (blue).

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Western Blot - Anti-Nucleoside-diphosphate kinase Antibody (A15278)

Immunoprecipitation analysis of 300µg extracts of HT-29 cells using 3µg of Anti-Nucleoside-diphosphate kinase Antibody (A15278). This Western blot was performed on the immunoprecipitate using Anti-Nucleoside-diphosphate kinase Antibody (A15278) at a dilution of 1:1000.

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