Anti-Nucleoside Phosphorylase Antibody (A85109)

Name: Anti-Nucleoside Phosphorylase Antibody
Brand: Antibodies.com
SKU: A85109
Price: 115.0 GBP
Availability: InStock

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$495

Goat polyclonal antibody to Nucleoside Phosphorylase for ELISA, WB and IHC.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-Nucleoside Phosphorylase Antibody

Description

Goat polyclonal antibody to Nucleoside Phosphorylase.

Applications

ELISA, WB, IHC

Dilutions

ELISA: 1:128,000, WB: 0.03-0.1µg/ml, IHC: 5µg/ml

Reactivity

Human

Immunogen

Synthetic peptide corresponding to the internal region, amino acids 142-153, of human Nucleoside Phosphorylase.

Sequence

C-SGQNPLRGPNDE

Host

Goat

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 µg/ml

Molecular Weight

30 kDa

Predicted MW

32.1kDa

Product Form

Liquid

Formulation

Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

Inosine phosphorylase, Inosine-guanosine phosphorylase, NP, PNP, Purine nucleoside phosphorylase

Isotype Controls

Goat IgG (A121671)

Suitable Secondaries

See all Anti-Goat IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (3)

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Western Blot - Anti-Nucleoside Phosphorylase Antibody (A85109)

Nucleoside Phosphorylase expression in Human Liver lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-Nucleoside Phosphorylase Antibody (A85109) at 0.1µg/ml and detected by chemiluminescence.

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Immunohistochemistry - Anti-Nucleoside Phosphorylase Antibody (A85109)

Nucleoside Phosphorylase expression in Human Tonsil analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by steaming in citrate buffer, pH 6. Staining was performed with Anti-Nucleoside Phosphorylase Antibody (A85109) at 5µg/ml and revealed with alkaline phosphatase (AP).

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Immunohistochemistry - Anti-Nucleoside Phosphorylase Antibody (A85109)

Nucleoside Phosphorylase expression in Human Kidney analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by steaming in citrate buffer, pH 6. Staining was performed with Anti-Nucleoside Phosphorylase Antibody (A85109) at 5µg/ml and revealed with alkaline phosphatase (AP).

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