Lyophilized from sterile Phosphate Buffered Saline, pH 7.4. Normally 5%–8% Trehalose is added as a protectant before lyophilization.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
General Notes
Prior to reconstitution, centrifuge the vial at 5,000g for 3-5 minutes at room temperature. Reconstitute with appropriate volume of distilled sterile water to bring product to 1mg/ml concentration. After addition of distilled sterile water, mix by gentle tapping. Note: It is not recommended to vortex or vigorously pipette the sample.
Immobilised Recombinant Human CD73 Protein (Fc Tag) (A317962) at 2 µg/ml (100 µl/well) can bind Oleclumab Biosimilar - Anti-CD73 Antibody (A332162) in a linear range of 0.13-16 ng/ml. In order to specifically detect Oleclumab Biosimilar - Anti-CD73 Antibody (A332162), mouse anti-human Fab-specific antibody was used as detection antibody.
CD73 expresion in Expi293 cells compared to irrelevant protein as analyzed by flow cytometry. Staining was performed with 1µg/ml of Oleclumab Biosimilar - Anti-CD73 Antibody to obtain the blue histogram or the red histogram for the irrelevant protein.
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