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Anti-Orexin Receptor 1/Ox-1-R Antibody (A90051)

Western Blot - Anti-Orexin Receptor 1/Ox-1-R Antibody (A90051) - Antibodies.com Immunofluorescence - Anti-Orexin Receptor 1/Ox-1-R Antibody (A90051) - Antibodies.com
$355
Product Datasheet

Rabbit polyclonal antibody to Orexin Receptor 1/Ox-1-R for WB and ICC/IF.

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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 7-10 business days.

  • Specifications

Name
Anti-Orexin Receptor 1/Ox-1-R Antibody
Description
Rabbit polyclonal antibody to Orexin Receptor 1/Ox-1-R.
Applications
WB, ICC/IF
Dilutions
WB: 1:500-1:1,000, ICC/IF: 1:50-1:200
Reactivity
Human, Mouse, Rat
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 150-250 of human HCRTR1 (NP_001516.2).
Sequence
HPLLFKSTARRARGSILGIWAVSLAIMVPQAAVMECSSVLPELANRTRLFSVCDERWADDLYPKIYHSCFFIVTYLAPLGLMAMAYFQIFRKLWGRQIPGT
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Concentration
Lot Specific
Molecular Weight
48 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (2)

Western Blot - Anti-Orexin Receptor 1/Ox-1-R Antibody (A90051) - Antibodies.comEnlarge Image

Western Blot - Anti-Orexin Receptor 1/Ox-1-R Antibody (A90051)

Western blot analysis of extracts of C6 cells, using Anti-Orexin Receptor 1/Ox-1-R Antibody (A90051) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.
Immunofluorescence - Anti-Orexin Receptor 1/Ox-1-R Antibody (A90051) - Antibodies.comEnlarge Image

Immunofluorescence - Anti-Orexin Receptor 1/Ox-1-R Antibody (A90051)

Immunofluorescence analysis of mouse brain using Anti-Orexin Receptor 1/Ox-1-R Antibody (A90051) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
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