Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
150 kDa oxygen-regulated protein, 170 kDa glucose-regulated protein, GRP-170, GRP170, Heat shock protein family H member 4, HSPH4, HYOU1, Hypoxia up-regulated protein 1, ORP-150
Western Blot - Anti-ORP150 Antibody [ARC1857] (A306853)
Western blot analysis of extracts of various cell lines, using Anti-ORP150 Antibody [ARC1857] (A306853) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.
Immunohistochemistry analysis of paraffin-embedded rat pancreas using Anti-ORP150 Antibody [ARC1857] (A306853) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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