Western blot analysis of extracts of Rat liver, using Anti-OTULIN Antibody (A305722) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 60s.
Western blot analysis of extracts of MCF7 cells, using Anti-OTULIN Antibody (A305722) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.
Immunofluorescence analysis of NIH/3T3 cells using Anti-OTULIN Antibody (A305722) at a dilution of 1:50 (40x lens). DAPI was used to stain the cell nuclei (blue).
Publishing research using Anti-OTULIN Antibody (A305722)? Please let us know so that we can list the citation on this page.
Proteins predicted to interact with OTULIN
Predicted protein interactions based upon String database. Revelancy score correlates with probability of interaction.
