Western Blot - Anti-p18 INK4c / CDKN2C Antibody (A92846)
Western blot analysis of extracts from normal (control) and CDKN2C/p18-INK4C knockout (KO) HeLa cells, using Anti-p18 INK4c / CDKN2C Antibody (A92846) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.