Anti-P4HA1 Antibody (A8644)

Name: Anti-P4HA1 Antibody
Brand: Antibodies.com
SKU: A8644
Price: 270.0 GBP
Availability: InStock

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$355

Rabbit polyclonal antibody to P4HA1 for WB and ICC/IF.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-P4HA1 Antibody

Description

Rabbit polyclonal antibody to P4HA1.

Applications

WB, ICC/IF

Dilutions

WB: 1:100-1:500, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 200-300 of human P4HA1 (NP_001017962.1).

Sequence

STIDKVSVLDYLSYAVYQQGDLDKALLLTKKLLELDPEHQRANGNLKYFEYIMAKEKDVNKSASDDQSDQKTTPKKKGVAVDYLPERQKYEMLCRGEGIKM

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

61 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

4-PH alpha-1, P4HA, Procollagen-proline,2-oxoglutarate-4-dioxygenase subunit alpha-1, Prolyl 4-hydroxylase subunit alpha-1

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (3)

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Western Blot - Anti-P4HA1 Antibody (A8644)

Western blot analysis of extracts of various cell lines, using Anti-P4HA1 Antibody (A8644) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 60s.

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Western Blot - Anti-P4HA1 Antibody (A8644)

Western blot analysis of extracts of Rat skeletal muscle, using Anti-P4HA1 Antibody (A8644) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 120.

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Immunofluorescence - Anti-P4HA1 Antibody (A8644)

Immunofluorescence analysis of L929 cells using Anti-P4HA1 Antibody (A8644) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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