Anti-P4HA3 Antibody (A90971)

Name: Anti-P4HA3 Antibody
Brand: Antibodies.com
SKU: A90971
Price: 270.0 GBP
Availability: InStock

Enlarge Image

$355

Rabbit polyclonal antibody to P4HA3 for WB and ICC/IF.

100% Guarantee

Price Match Guarantee

Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-P4HA3 Antibody

Description

Rabbit polyclonal antibody to P4HA3.

Applications

WB, ICC/IF

Dilutions

WB: 1:1,000-1:5,000, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 20-110 of human P4HA3 (NP_878907.1).

Sequence

DPERAAARGDTFSALTSVARALAPERRLLGLLRRYLRGEEARLRDLTRFYDKVLSLHEDSTTPVANPLLAFTLIKRLQSDWRNVVHSLEAS

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

70 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

4-PH alpha-3, Procollagen-proline,2-oxoglutarate-4-dioxygenase subunit alpha-3, Prolyl 4-hydroxylase subunit alpha-3

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (3)

Enlarge Image

Western Blot - Anti-P4HA3 Antibody (A90971)

Western blot analysis of extracts of 293T cells, using Anti-P4HA3 Antibody (A90971) at 1:3,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

Enlarge Image

Western Blot - Anti-P4HA3 Antibody (A90971)

Western blot analysis of extracts of various cell lines, using Anti-P4HA3 Antibody (A90971) at 1:3,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 90s.

Enlarge Image

Immunofluorescence - Anti-P4HA3 Antibody (A90971)

Immunofluorescence analysis of NIH/3T3 cells using Anti-P4HA3 Antibody (A90971) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).

Publishing research using Anti-P4HA3 Antibody (A90971)? Please let us know so that we can list the citation on this page.