MDA-MB-231 cells and breast or colon carcinoma tissue.
Dilutions
Flow Cytometry: 1-2 µg/million cells, IF: 1-2 µg/mL, WB: 1-2 µg/mL, IHC-P: 1-2 µg/mL. Directly conjugated primary antibodies may require increased working concentrations compared with indirect detection systems. Antibody concentration and assay conditions should be optimized for each application. A suggested starting concentration is 1-2 µg/mL for most assays.
Reactivity
Human, Monkey, Bovine
Immunogen
Recombinant human wild type p53 protein expressed in E. coli.
Host
Mouse
Clonality
Monoclonal
Clone ID
DO-7
Isotype
IgG2b
Light Chains
kappa
Conjugate
CF®740
Concentration
0.1 mg/mL
Predicted MW
53 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 0.1% recombinant BSA (produced in CHO cells) and 0.05% Sodium Azide.
Storage
Shipped and stored at +4°C. This product is also photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. This CF® conjugated antibody is guaranteed for 2 years from data of receipt when stored correctly.
