Anti-PAC1R Antibody (A334483)

Immunohistochemical detection of PACAP Receptor 1 (PAC1) in mouse spinal cord dorsal horn. Spinal cord sections were dewaxed, microwaved in citric acid, and stained with anti-PAC1R antibody (A334483) at 1:100, followed by biotinylated anti-rabbit IgG and avidin-biotin solution. Sections were developed with 3,3-diaminobenzidine (DAB)-glucose oxidase and lightly counterstained with hematoxylin. PAC1 receptors were uniformly detected at the plasma membrane of nearly all cells.

Immunocytochemical analysis of PACAP Receptor 1 (PAC1) in HEK293 cells. HEK293 cells stably expressing PAC1 were untreated or treated with 1 µM PACAP for 30 min and stained with anti-PAC1R antibody (A334483) at 1:200. PAC1 receptors were confined to the plasma membrane in untreated cells (0 min) and observed in perinuclear vesicular clusters after 30 min of PACAP exposure.

Western blot confirmation of PACAP Receptor 1 (PAC1) in transfected HEK293 cells. Lysates from native HEK293 cells (MOCK) or cells stably expressing mouse PAC1 were probed with phosphorylation-independent anti-PAC1 antibody (A334483) at 1:1000.