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Recombinant Anti-pan Histone H3 Antibody [RM188] (A121319)

Multiplex Immunoassay - Anti-pan Histone H3 Antibody [RM188] (A121319) - Antibodies.com Western Blot - Anti-pan Histone H3 Antibody [RM188] (A121319) - Antibodies.com Immunocytochemistry - Anti-pan Histone H3 Antibody [RM188] (A121234) - Antibodies.com ELISA - Anti-pan Histone H3 Antibody [RM188] (A121319) - Antibodies.com ELISA - Anti-pan Histone H3 Antibody [RM188] (A121319) - Antibodies.com
Recombinant Antibody
$585
Product Datasheet

Recombinant rabbit monoclonal [RM188] antibody to pan Histone H3 for WB, ELISA, Multiplex Protein Detection and ICC.

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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 5-8 business days.

  • Specifications

Name
Anti-pan Histone H3 Antibody [RM188]
Description
Recombinant rabbit monoclonal [RM188] antibody to pan Histone H3.
Specificity
This antibody recognizes all Histone H3 proteins, independent of post-translational modifications.
Applications
WB, ELISA, Multiplex Protein Detection, ICC
Dilutions
WB: 0.01-0.25 µg/ml, ICC: 1-5 µg/ml, ELISA: 0.2-1 µg/ml, Multiplex Protein Detection: 0.1-0.5 µg/ml
Reactivity
All Vertebrates
Cross Reactivity
There is no cross reactivity with other Histone proteins.
Immunogen
Synthetic peptide corresponding to human pan Histone H3 (C terminal).
Host
Rabbit
Clonality
Monoclonal
Clone ID
RM188
Isotype
IgG
Conjugate

Unconjugated

Purification
Protein A chromatography.
Concentration
1 mg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 50% Glycerol, 1% BSA, and 0.09% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (5)

Multiplex Immunoassay - Anti-pan Histone H3 Antibody [RM188] (A121319) - Antibodies.comEnlarge Image

Anti-pan Histone H3 Antibody [RM188] (A121319)

Anti-pan Histone H3 Antibody [RM188] reacts to both Histone H3.1 and Histone H3.3.
Western Blot - Anti-pan Histone H3 Antibody [RM188] (A121319) - Antibodies.comEnlarge Image

Western Blot - Anti-pan Histone H3 Antibody [RM188] (A121319)

Western blot of recombinant Histone H3.3 and Histone H3.1 proteins and A375, HEK293, HeLa, and SK-MEL-2 whole cell lysates, using Anti-pan Histone H3 Antibody [RM188] at 0.025µg/ml, showed a band of recombinant H3.1 and H3.3 proteins, and total Histone H3 in A375, HEK293, HeLa, and SK-MEL-2 cells.
Immunocytochemistry - Anti-pan Histone H3 Antibody [RM188] (A121234) - Antibodies.comEnlarge Image

Immunocytochemistry - Anti-pan Histone H3 Antibody [RM188] (A121319)

Immunocytochemistry of HeLa cells, using Anti-pan Histone H3 Antibody [RM188] (red). Actin filaments have been labeled with fluorescein phalloidin (green).
ELISA - Anti-pan Histone H3 Antibody [RM188] (A121319) - Antibodies.comEnlarge Image

Anti-pan Histone H3 Antibody [RM188] (A121319)

Sandwich ELISA against acetylated Histone H3 at Lys9 using HeLa whole cell lysate, treated or untreated with Sodium Butyrate. 1µg/ml of Anti-pan Histone H3 Antibody [RM188] was used as the capture antibody and 1µg/ml of Anti-Histone H3 (acetyl Lys9) Antibody [RM161] (Biotin) was used as the detection antibody.
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