Western Blot - Anti-pan Ubiquitin Remnant Motif (K-e-GG) Antibody (A309793)
Western blot analysis of extracts of various cell lines, using Anti-pan Ubiquitin Remnant Motif (K-e-GG) Antibody (A309793) at 1:1,000 dilution. HeLa cells were treated by MG132(50 µM) at 37°C for 90 minutes. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 180s.
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