This antibody specifically recognizes the C-terminal domain of human PAR2, detecting the non-phosphorylated form. It is validated for Western blot analysis, enables immunoprecipitation from cell and tissue lysates, and is suitable for immunohistochemistry in cultured cells and tissue sections.
Applications
WB, IHC
Dilutions
WB: 1,000, IHC: 1:100
Reactivity
Human
Immunogen
Synthetic peptide corresponding to the C-terminal of human PAR2. Immunogen range is 22-22 amino acids.
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Purification
Antigen affinity purification.
Concentration
Lot Specific
Product Form
Liquid
Formulation
Supplied in Dulbecco's PBS, pH 7.4, with 150 mM NaCl and 0.005% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Western blot confirmation of Proteinase-Activated Receptor 2 (PAR2) in transfected HEK293 cells. Lysates from native HEK293 cells (MOCK) or cells stably expressing PAR2 were immunoblotted with anti-PAR2 antibody (A334538) at 1:1000.
Immunohistochemical localization of Proteinase-Activated Receptor 2 (PAR2) in human kidney. Kidney sections were dewaxed, microwaved in citric acid, and stained with anti-PAR2 antibody (A334538) at 1:100, followed by biotinylated anti-rabbit IgG and avidin-biotin solution. Sections were developed with 3,3-diaminobenzidine (DAB)-glucose oxidase and lightly counterstained with hematoxylin.
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