Parkin expression in various lysates analyzed by western blot. Primary antibody incubation was performed for 1 hour on 25ug protein per lane with Anti-Parkin Antibody (A80577) at a dilution of 1:1000 and detected with chemiluminescence.
Parkin expression in rat kidney tissue analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved with 10 mM citrate buffer, pH 6.0, under high pressure. Staining was performed with Anti-Parkin Antibody (A80577) at a dilution of 1:200.
Parkin expression in mouse kidney tissue analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved with 10 mM citrate buffer, pH 6.0, under high pressure. Staining was performed with Anti-Parkin Antibody (A80577) at a dilution of 1:200.
Parkin expression in C6 cells analyzed by immunofluorescence. Staining was performed with Anti-Parkin Antibody (A80577) at a dilution of 1:50 followed by Cy3-conjugated Goat anti-Rabbit IgG (H+L) secondary antibody at a dilution of 1:500. Nuclei were stained with DAPI (blue).
Parkin expression in NIH/3T3 cells analyzed by immunofluorescence. Staining was performed with Anti-Parkin Antibody (A80577) at a dilution of 1:50 followed by Cy3-conjugated Goat anti-Rabbit IgG (H+L) secondary antibody at a dilution of 1:500. Nuclei were stained with DAPI (blue).
