Anti-PCB Antibody (A15157)

Name: Anti-PCB Antibody
Brand: Antibodies.com
SKU: A15157
Price: 270.0 GBP
Availability: InStock

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$355

Rabbit polyclonal antibody to PCB for WB, ICC/IF and IP.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-PCB Antibody

Description

Rabbit polyclonal antibody to PCB.

Applications

WB, ICC/IF, IP

Dilutions

WB: 1:500-1:1,000, ICC/IF: 1:10-1:100, IP: 1:500-1:1,000

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 879-1178 of human Pyruvate carboxylase (Pyruvate carboxylase (PC)) (NP_001035806.1).

Sequence

HSMGLGSKFKEVKKAYVEANQMLGDLIKVTPSSKIVGDLAQFMVQNGLSRAEAEAQAEELSFPRSVVEFLQGYIGVPHGGFPEPFRSKVLKDLPRVEGRPGASLPPLDLQALEKELVDRHGEEVTPEDVLSAAMYPDVFAHFKDFTATFGPLDSLNTRLFLQGPKIAEEFEVELERGKTLHIKALAVSDLNRAGQRQVFFELNGQLRSILVKDTQAMKEMHFHPKALKDVKGQIGAPMPGKVIDIKVVAGAKVAKGQPLCVLSAMKMETVVTSPMEGTVRKVHVTKDMTLEGDDLILEIE

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

130 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

PC, Pyruvate carboxylase, mitochondrial, Pyruvic carboxylase

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (3)

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Western Blot - Anti-PCB Antibody (A15157)

Western blot analysis of various lysates, using Anti-PCB Antibody (A15157) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3s.

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Immunofluorescence - Anti-PCB Antibody (A15157)

Immunofluorescence analysis of U2OS cells using Anti-PCB Antibody (A15157). DAPI was used to stain the cell nuclei (blue).

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Western Blot - Anti-PCB Antibody (A15157)

Immunoprecipitation analysis of 300µg extracts of HeLa cells using 3µg of Anti-PCB Antibody (A15157). This Western blot was performed on the immunoprecipitate using Anti-PCB Antibody (A15157) at a dilution of 1:1000.

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