Anti-PCMT1 Antibody (A12005)

Name: Anti-PCMT1 Antibody
Brand: Antibodies.com
SKU: A12005
Price: 270.0 GBP
Availability: InStock

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$355

Rabbit polyclonal antibody to PCMT1 for WB, ICC/IF and IP.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-PCMT1 Antibody

Description

Rabbit polyclonal antibody to PCMT1.

Applications

WB, ICC/IF, IP

Dilutions

WB: 1:500-1:2,000, ICC/IF: 1:50-1:100, IP: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 62-286 of human PCMT1 (NP_001238978.1).

Sequence

KSGGASHSELIHNLRKNGIIKTDKVFEVMLATDRSHYAKCNPYMDSPQSIGFQATISAPHMHAYALELLFDQLHEGAKALDVGSGSGILTACFARMVGCTGKVIGIDHIKELVDDSVNNVRKDDPTLLSSGRVQLVVGDGRMGYAEEAPYDAIHVGAAAPVVPQALIDQLKPGGRLILPVGPAGGNQMLEQYDKLQDGSIKMKPLMGVIYVPLTDKEKQWSRDEL

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

25 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

L-isoaspartyl protein carboxyl methyltransferase, PIMT, Protein L-isoaspartyl/D-aspartyl methyltransferase, Protein-beta-aspartate methyltransferase, Protein-L-isoaspartate(D-aspartate) O-methyltransferase

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (3)

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Western Blot - Anti-PCMT1 Antibody (A12005)

Western blot analysis of extracts of various cell lines, using Anti-PCMT1 Antibody (A12005). The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Immunofluorescence - Anti-PCMT1 Antibody (A12005)

Immunofluorescence analysis of HeLa cells using Anti-PCMT1 Antibody (A12005). DAPI was used to stain the cell nuclei (blue).

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Western Blot - Anti-PCMT1 Antibody (A12005)

Immunoprecipitation analysis of 200µg extracts of HepG2 cells using 1µg of Anti-PCMT1 Antibody (A12005). This Western blot was performed on the immunoprecipitate using Anti-PCMT1 Antibody (A12005) at a dilution of 1:1000.

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