Mouse monoclonal [PC10] antibody to PCNA (CF®594).
Applications
Flow Cytometry (Intracellular), IHC, IHC-P
Positive Control
Tonsil or reactive lymph node tissue.
Dilutions
Flow Cytometry: 0.5-1 µg/million cells in 100 µL, IF: 0.5-1 µg/mL, WB: 0.5-1 µg/mL, IHC-P: 0.25-0.5 µg/mL. For IHC-P, heat-induced epitope retrieval is recommended using 10 mM citrate buffer, pH 6.0, for 10-20 minutes, followed by cooling to room temperature for 20 minutes. Directly conjugated primary antibodies may require higher working concentrations than indirect detection systems.
Supplied in Phosphate Buffered Saline with 0.1% BSA and 0.05% Sodium Azide.
Storage
Shipped and stored at +4°C. This product is also photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. This CF® conjugated antibody is guaranteed for 2 years from data of receipt when stored correctly.
Synonyms
Cyclin, Proliferating cell nuclear antigen
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Publishing research using Anti-PCNA Antibody [PC10] (CF®594) (A352555)? Please let us know so that we can list the citation on this page.
Alternative products to Anti-PCNA Antibody [PC10] (CF®594) (A352555)