Mouse monoclonal [PDL1/2743] antibody to PD-L1 (Biotin).
Applications
Flow Cytometry, IF
Positive Control
Heart, placenta, spleen or tonsil tissue. Jurkat cells (IF/FACS).
Dilutions
Flow Cytometry: 1-2 µg/mL, IF: 1-2 µg/mL. For ELISA coating applications, use a BSA-free antibody formulation. Directly conjugated primary antibodies may require higher working concentrations than indirect detection systems.
Reactivity
Human
Immunogen
Recombinant fragment of human PD-L1 (CD274) protein corresponding to approximately amino acids 39-191 (exact sequence is proprietary).
Host
Mouse
Clonality
Monoclonal
Clone ID
PDL1/2743
Isotype
IgG2b
Light Chains
kappa
Conjugate
Biotin
Concentration
0.1 mg/mL
Predicted MW
37-50 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 0.1% BSA and 0.05% Sodium Azide.
Storage
Shipped and stored at +4°C. This product is also photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. This CF® conjugated antibody is guaranteed for 2 years from data of receipt when stored correctly.
Immunofluorescence of formaldedhyde-fixed Jurkat cells stained with unconjugated Anti-PD-L1 Antibody [PDL1/2743] (A248770) followed by Goat Anti-Mouse IgG H&L Antibody (CF®488A) (A343429; green). Nuclei were counterstained with RedDot™2 (red).
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