Anti-PD1 Antibody [4F12] (A279378)

Anti-PD1 Antibody [4F12]

Mouse monoclonal [4F12] antibody to PD1.

This antibody recognises human CD279, otherwise known as PD-1/PDCD1, a type I transmembrane glycoprotein and member of the Ig superfamily, which acts as a co-stimulatory molecule and negative regulator of T-cell activation. CD279 is an immunoinhibitory receptor which interacts with its ligands CD274 (PD-L1) and CD273 (PD-L2) to inhibit the activation and proliferation of T and B cells. CD279 is associated with regulating peripheral tolerance of T cells and preventing autoimmunity and autoimmune diseases, including type I diabetes and rheumatoid arthritis.

ELISA, WB

WB: 1:500 - 1:1,000

Human

Recombinant human CD279 (amino acids 21-167) purified from E. coli.

PGWFLDSPDRPWNPPTFSPALLVVTEGDNATFTCSFSNTSESFVLNWYRMSPSNQTDKLAAFPEDRSQPGQDCRFRVTQLPNGRDFHMSVVRARRNDSGTYLCGAISLAPKAQIKESLRAELRVTERRAEVPTAHPSPSPRPAGQFQ

Mouse

Monoclonal

4F12

IgG2b

1 mg/ml

Approximately 32-36 kDa in 293T and U937 cell lysates.

Liquid

Supplied in Phosphate Buffered Saline with 10% Glycerol and 0.02% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Mouse anti Human CD279, clone 4F12 recognizes human CD279, otherwise known as PD-1/PDCD1, a type I transmembrane glycoprotein and member of the Ig superfamily, which acts as a co-stimulatory molecule and negative regulator of T-cell activation (Patsoukis et al. 2012).CD279 is an immunoinhibitory receptor which interacts with its ligands CD274 (PD-L1) and CD273 (PD-L2) to inhibit the activation and proliferation of T and B cells. CD279 is associated with regulating peripheral tolerance of T cells and preventing autoimmunity and autoimmune diseases, including type I diabetes and rheumatoid arthritis (Okazaki et al. 2005).

CD279, CD279 antigen, hPD 1, hPD l, hPD-1, hSLE1, PD 1, PD-1, PDCD 1, PDCD1, PDCD1_HUMAN, Programmed cell death 1, Programmed cell death 1 protein, Programmed cell death protein 1, Protein PD 1, Protein PD-1, SLEB2, Systemic lupus erythematosus susceptibility 2