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Anti-PD1 Antibody (A9719)

Western Blot - Anti-PD1 Antibody (A9719) - Antibodies.com Western Blot - Anti-PD1 Antibody (A9719) - Antibodies.com
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$485
Product Datasheet

Rabbit polyclonal antibody to PD1 for WB.

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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 7-10 business days.

  • Specifications

Name
Anti-PD1 Antibody
Description
Rabbit polyclonal antibody to PD1.
Applications
WB
Dilutions
WB: 1:100-1:500
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant fusion protein containing a sequence within amino acids 1-80 of human PD-1/CD279 (NP_005009.2).
Sequence
MQIPQAPWPVVWAVLQLGWRPGWFLDSPDRPWNPPTFSPALLVVTEGDNATFTCSFSNTSESFVLNWYRMSPSNQTDKLA
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Concentration
Lot Specific
Molecular Weight
35 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
CD279, CD279 antigen, hPD 1, hPD l, hPD-1, hSLE1, PD 1, PD-1, PDCD 1, PDCD1, PDCD1_HUMAN, Programmed cell death 1, Programmed cell death 1 protein, Programmed cell death protein 1, Protein PD 1, Protein PD-1, SLEB2, Systemic lupus erythematosus susceptibility 2
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (2)

Western Blot - Anti-PD1 Antibody (A9719) - Antibodies.comEnlarge Image

Western Blot - Anti-PD1 Antibody (A9719)

Western blot analysis of extracts of various cell lines, using Anti-PD1 Antibody (A9719) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.
Western Blot - Anti-PD1 Antibody (A9719) - Antibodies.comEnlarge Image

Western Blot - Anti-PD1 Antibody (A9719)

Western blot analysis of extracts from normal (control) and PD-1/CD279 knockout (KO) 293T cells, using Anti-PD1 Antibody (A9719) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.
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