Anti-PDGFR alpha Antibody [35248 (57/20)] (A279264)

Anti-PDGFR alpha Antibody [35248 (57/20)]

Mouse monoclonal [35248 (57/20)] antibody to PDGFR alpha.

This antibody recognises the human platelet-derived growth factor receptor alpha, a 1089 amino acid, ~180 kDa single pass type I transmembrane glycoprotein.PDGF is a major serum mitogen that can exist as a homo- or heterodimeric protein consisting of disulphide-linked PDGF-A and PDGF-B chains. The PDGF-AA, PDGF-BB and PDGF-AB isoforms bind to two distinct cell surface PDGF receptors with different affinities. Whereas PDGF receptor alpha binds all three isoforms with high affinity, PDGF R beta binds PDGF-BB and AB but not PDGF-AA. Both PDGF receptor alpha and beta are members of the class III subfamily of receptor tyrosine kinases (Westermark 1995). Mouse anti Human CD140a antibody, clone 35248 is suitable for use in functional assays. The ND50 was determined as 0.2-0.6 µg/ml for blocking 10 ng/ml of PDGF-AA biological activity on a quiescent confluent cell culture of human WS1 cells.

WB, IHC-P, ELISA, IF

ELISA: 0.5 µg/ml - 1 µg/ml, IHC-P: 10 µg/ml - 20 µg/ml, WB: 1 µg/ml - 2 µg/ml

Human

Purified recombinant human platelet-derived growth factor (PDGF) receptor alpha (extracellular domain), derived from the insect cell line Sf21.

Mouse

Monoclonal

35248 (57/20)

IgG1

1 mg/ml

Liquid

Supplied in Phosphate Buffered Saline with 5% Trehalose and <0.1% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Mouse anti Human CD140a antibody, clone 35248 recognizes the human platelet-derived growth factor receptor alpha, a 1089 amino acid, ~180 kDa single pass type I transmembrane glycoprotein.PDGF is a major serum mitogen that can exist as a homo- or heterodimeric protein consisting of disulphide-linked PDGF-A and PDGF-B chains (Hart et al. 1990). The PDGF-AA, PDGF-BB and PDGF-AB isoforms bind to two distinct cell surface PDGF receptors with different affinities. Whereas PDGF receptor alpha binds all three isoforms with high affinity, PDGF R beta binds PDGF-BB and AB but not PDGF-AA (Hart et al. 1988). Both PDGF receptor alpha and beta are members of the class III subfamily of receptor tyrosine kinases (Westermark 1995).Mouse anti Human CD140a antibody, clone 35248 is suitable for use in functional assays. The ND50 was determined as 0.2-0.6 µg/ml for blocking 10 ng/ml of PDGF-AA biological activity on a quiescent confluent cell culture of human WS1 cells.

Alpha platelet-derived growth factor receptor, Alpha-type platelet-derived growth factor receptor, CD140 antigen-like family member A, CD140a, CD140a antigen, PDGF-R-alpha, PDGFR-2, PDGFR-alpha, PDGFR2, PDGFRA, Platelet-derived growth factor alpha receptor, Platelet-derived growth factor receptor 2, Platelet-derived growth factor receptor alpha, RHEPDGFRA