Anti-PDHA1 Antibody [ARC0722] (A308220)

Name: Anti-PDHA1 Antibody [ARC0722]
Brand: Antibodies.com
SKU: A308220
Price: 325.0 GBP
Availability: InStock

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$485

Rabbit monoclonal [ARC0722] antibody to PDHA1 for WB and ICC/IF.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-PDHA1 Antibody [ARC0722]

Description

Rabbit monoclonal [ARC0722] antibody to PDHA1.

Applications

WB, ICC/IF

Dilutions

WB: 1:500-1:2,000, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 50-150 of human PDHA1 (P08559).

Sequence

PPVTTVLTREDGLKYYRMMQTVRRMELKADQLYKQKIIRGFCHLCDGQEACCVGLEAGINPTDHLITAYRAHGFTFTRGLSVREILAELTGRKGGCAKGKG

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC0722

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

43 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

PDHE1-A type I, PHE1A, Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (2)

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Western Blot - Anti-PDHA1 Antibody [ARC0722] (A308220)

Western blot analysis of extracts of various cell lines, using Anti-PDHA1 Antibody [ARC0722] (A308220) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3s.

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Immunofluorescence - Anti-PDHA1 Antibody [ARC0722] (A308220)

Immunofluorescence analysis of NIH-3T3 cells using Anti-PDHA1 Antibody [ARC0722] (A308220) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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