Western Blot - Anti-PDPK1 (phospho Ser241) Antibody (A16648)
Western blot analysis of extracts from 293 cells, using Anti-PDPK1 (phospho Ser241) Antibody (A16648). The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using Anti-PDPK1 (phospho Ser241) Antibody (A16648). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.