Anti-PEA15 Antibody [4D2] (A270553)

Immunofluorescent analysis of rat brain section stained with Anti-PEA15 Antibody [4D2] (A270553), at a dilution of 1:1,000, in red, and co-stained with Anti-GFAP Antibody (A85419), at a dilution of 1:5,000, in green. Nuclear DNA is visualised in blue using Hoechst staining. Following transcardial perfusion with 4% paraformaldehyde, the brain was post-fixed for 24 hours, cut to 45 µm, and free-floating sections were stained using the above antibodies. Anti-PEA15 Antibody [4D2] (A270553) labels the cytoplasm of certain cells which are not labelled by the astrocyte specific Anti-GFAP Antibody (A85419).

Western blot analysis of different tissue lysates using Anti-PEA15 Antibody [4D2] (A270553), at a dilution of 1:1,000, in green. The lanes contain: [Lane 1] protein standard (red), [Lane 2] rat whole brain, [Lane 3] rat cerebellum, [Lane 4] mouse whole brain, [Lane 5] cow cortex, and [Lane 6] cow cerebellum. The single strong band at about 15 kDa corresponds to the PEA15 protein.