Anti-PHAPI2/APRIL Antibody [ARC2014] (A307147)

Western blot analysis of extracts of various cell lines, using Anti-PHAPI2/APRIL Antibody [ARC2014] (A307147) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3s.

Immunohistochemistry analysis of paraffin-embedded rat spleen using Anti-PHAPI2/APRIL Antibody [ARC2014] (A307147) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry analysis of paraffin-embedded human thyroid cancer using Anti-PHAPI2/APRIL Antibody [ARC2014] (A307147) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry analysis of paraffin-embedded mouse kidney using Anti-PHAPI2/APRIL Antibody [ARC2014] (A307147) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.