Western Blot - Anti-PHGDH/Malate dehydrogenase Antibody (A12950)
Western blot analysis of extracts of various cell lines, using Anti-PHGDH/Malate dehydrogenase Antibody (A12950) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.
Immunofluorescence analysis of HeLa cells using Anti-PHGDH/Malate dehydrogenase Antibody (A12950) at a dilution of 1:300 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunofluorescence analysis of NIH/3T3 cells using Anti-PHGDH/Malate dehydrogenase Antibody (A12950) at a dilution of 1:300 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunofluorescence analysis of PC-12 cells using Anti-PHGDH/Malate dehydrogenase Antibody (A12950) at a dilution of 1:300 (40x lens). DAPI was used to stain the cell nuclei (blue).
Western Blot - Anti-PHGDH/Malate dehydrogenase Antibody (A12950)
Immunoprecipitation analysis of 300µg extracts of HeLa cells using 3µg of Anti-PHGDH/Malate dehydrogenase Antibody (A12950). This Western blot was performed on the immunoprecipitate using Anti-PHGDH/Malate dehydrogenase Antibody (A12950) at a dilution of 1:1000.
